Clearly, in the second scenario, using more pools decreases the risk of missing a real rare sequence variant.
Of course, although complete genome sequences are not available and RT-PCR results can artifactually miss a divergent sequence, these data collectively suggest that an unique event of loss of AR-B occurred early on in the Otophysi lineage.
When superpositioning homologous proteins with different sequences or identical proteins with missing portions, a sequence alignment must be provided to THESEUS.
(I should note, in fairness, that thanks to the vicissitudes of transcontinental travel, I missed a short sequence at the beginning of this 90-minute work on Saturday evening).
Other results showed that recombination in U3 dropped significantly in tests conducted with a template construct missing a 150-nt long sequence containing the putative dimerization site at the 3′ end, suggesting the existence of sequence elements in this region that are crucial for efficient homologous recombination in this region.
Because most reads covering the ends of exons will also span splice junctions, the ends of exons in the pseudoconsensus will initially be covered by few reads, and as a result, an exon's pseudoconsensus will likely be missing a small amount of sequence on each end.
In addition, the two Langdon sequences share differences to the two Norin 61 sequences at four positions, i.e., the Langdon sequences are missing a glutamic acid (E) codon found in both Norin 61 sequences at position 35, and differences in amino acids exist at positions 128, 376, and 435.
All other sequences missing a signal peptide appear to be incomplete, based on the absence of an N-terminal methionine and, therefore, could carry targeting signals.
The second bat CD94 sequence is missing a conserved cysteine in the extracellular domain that forms an intrachain disulphide bond.
The anticipated reason behind this is accounted to short sequence reads missing a MID from either end.
