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Some pairs, as in Fig. 2d, had only one cell missing a micronucleus.
Further, the number of cells missing a micronucleus increased with time.
At early times after treatment with colchicine, the majority of pairs had only one cell missing a micronucleus.
With time, cells began to lose their micronuclei, and by five hours more than half of the paired cells had at least one cell missing a micronucleus.
Initially many more pairs had one cell missing a micronucleus while a micronucleus was clearly visible in the other cell (e.g. Fig. 2d).
The surprise observation was that after some time elapsed, some cells in pairs were observed to be missing a micronucleus; based on the absence of a DAPI stained structure, the micronucleus was lost (Fig. 2d).
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