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In the current study, one missense mutant, msh2 -P689L, was classified as a pseudo-wild type based on the fluctuation assays, whereas the remaining missense strains were indistinguishable from the null allele (Table 1).
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CD36 has a missense variant.
Rates of misreading were calculated by dividing the ratio of firefly luciferase activity to Renilla luciferase activity generated from the missense vector in strains harboring the indicated mutant allele by the ratio generated with the sense plasmid.
Of these 62 strains, missense mutations were detected in QRDR of gyrA (n = 52) and parE (n = 1).
In several SAMP strains, missense mutations were detected in the Prx, Ldb3, and Gja3 genes, which mutations have been found in various human degenerative diseases.
Mutations were not detected in fabF, but the resistant strains harbored missense mutations in fabH.
Three NBCCS fibroblast strains bore missense mutations and 3 bore nonsense mutations in the PTCH1 gene.
Nonsense mutations leading to a premature stop codon and truncation of the predicted protein product were found in 25 strains and missense mutations leading to an amino acid change were found in 27 strains.
PBF2 has nine missense mutations between the BY and RM strains and is differentially expressed between the strains that have the BY allele and those that have the RM allele (p-value = 3.0e-2).
In this strain, a missense mutation of the leptin protein changing the valine at position 145 to a glutamate (p.V145E) was detected, which was caused by a transversion of the thymine at position 12971 of the leptin gene to an adenine (g.12971T>A) corresponding to position 434 of the transcript (c.434T>A) [EnsemblsENSMUSG0000005920159201, Ensembl:ENSMUST00000069789].
We considered the sequence of the RM11-1a stragainstinsthehe S288c strain and found five missense mutations in the EST2 transcript (EST2 was not differentially expressed between the two strains, p-value > .2), including an R to Q mutation in the reverse transcriptase domain.
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