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Furthermore, this system may potentially miss interactions involving proteins with significant hydrophobic domains such as membrane proteins, which may not be folded correctly.
Presumably, the robust nature of the genetic interaction data reflects the in vivo as opposed to the in vitro nature of proteomic determinations that tend to miss interactions with loosely associated proteins or misidentify interactions with overly abundant proteins [104].
They also miss interactions that depend on specific protein modifications that do not occur in the context of the yeast cell.
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While such approaches are effective in reducing the impact of spuriously-detected and missing interactions, they do not directly address transient interactions and non-interacting complex proteins.
Therefore, in order to avoid missing interactions in other compartments, BiFC was used to further verify the interacting partners after Y2H identification in the present investigation.
As such, it may impede potentially crucial theoretical advances stemming from missed interactions.
Twitter has been exploring the promises of the algorithmic feed for quite some time, but it's opted to push most of these minor updates to the Explore feed or just to the top of users' main feeds with brief "what you missed" interactions.
Therefore, it is possible that some expected yet missing interactions may represent an underestimate by the not so much data.
The abundance of missing interactions makes the normalization of the interaction overlap impractical.
Identified interactions are shown by ✓ and cells marked by ✘ indicate missed interactions by a method.
Adjacent vertices not connected by an edge represent "missing" interactions (e.g. v5 − v6).
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