Sentence examples for mismatched substrates from inspiring English sources

The archaeal NucS binds and cleaves both strands at dsDNA mismatched substrates, with the mispaired bases in the central position, leaving 5-nucleotide long 5′-cohesive ends12.

Moreover, theoretical analysis of the elastic energy relaxation in NWs of different geometries grown on lattice mismatched substrates was performed assuming an exponential decay of strain [9].

Semiconductor single-crystalline nanowires (NWs) or nanobelts (NBs) can be grown on lattice mismatched substrates and constructed into devices with the bottom-up method on basically any substrates [1].

Base-pairing of NTPs to the DNA template can provide differences in affinity between matched and mismatched substrates, but not between matched NTPs and matched dNTPs (which can base-pair as well).

Working with the ATP-dependent DNA ligase from the hyperthermophilic archaeon, Thermococcus kodakaraensis, Nakatani et al [ 27] found that the enzyme could seal substrates with mismatched base-pairing at the 5' end of the nick, but did not show activity towards the 3' mismatched substrates.

Regarding cleavage of mismatched substrates, no significant specific cleavage activity was observed on different substrates containing a single nucleotide mismatch in the central region of the strand (Supplementary Fig. 1B), in contrast with previous results obtained with the archaeal T. kodakarensis NucS12.

For nuclease activity, fluorescein-labelled 36-mer DNA was annealed to a fully complementary opposite strand (control) or carrying a single nucleotide mismatch in the central region of the strand (mismatch substrates).

How is the wide range of mismatch substrates recognized by CEL I? What determines the pH optima for RNase and DNase activities [ 7, 9, 29]?

Also, the 0-mismatch substrate shows unusual fluctuations in mutation frequency.

Unusual patterns are also seen when comparing the single and double BER knockdowns, and the patterns seen for PCNA and ATR are hard to interpret, again because of the fluctuation in the 0-mismatch substrate.

We report on the significance of thermal expansion mismatch in the heteroepitaxial growth of CdTe on highly lattice-mismatched substrates.