Sentence examples for mismatched position from inspiring English sources

That is, each unstable end and mismatched base pair may destabilize hybridization of three or four neighboring base pairs, preventing hybridization of whole base pairs between the end and mismatched position.

Six the remaining clones had the potential to form either a weak stem structure or a stem with a single mismatched position.

Of particular interest, our results indicated that the effect of mismatched position on the behavior of duplex formation changes according to probe length.

Although this speculation is also supported by the observation that the PM/MM ratios of the mismatched positions 6 8 from the end showed the best specificity with most probe lengths, further studies are necessary to understand the effects of the mismatched position on hybridization behavior on microarrays.

However, setting "–e" at 20 only allows extremely low quality mismatched positions, and therefore, rules out the majority of reads with high quality mismatched positions.

The effects of random noise can always be reduced by replicated measurements, whilst the systematic effects due to mismatch position and type cannot.

Indeed, the observed sensitivity to mismatch position of Affymetrix 25 mer probes has been exploited for SNP detection in applications such as SNPscanner [ 15].

If sequences have mismatch less than e, then mismatch positions is denoted in m l as 'n' instead of the original 'C', 'T', 'C' or 'G'.

For each basic 25-mer probe described above, we generated various probes by changing their length, mismatch position, and type of mismatched nucleotide as follows.

As mentioned in the above section "Design of the array," we provided all possible substitutions, i.e., each mismatch position and each type of mismatched nucleotide in every probe length, to investigate mismatch conditions comprehensively.

The present assay can overcome to two main problems of the hybridization biosensors, direct electron transfer of the redox reporter with the electrode surface and the positioning of the reporter before mismatch position.