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Exact(4)
A single bp mismatch was permitted and reads with multiple alignments were discarded.
To promise the quality for mapping reads, only one nucleotide mismatch was permitted for all sequence alignments.
All high quality tags were mapped to the reference sequence generated by transcriptome sequencing, and only 1 bp mismatch was permitted.
RNAhybrid and SMVLight were used to predict the targets of the miRNAs (p value < 0.05, Prediction-value > 1, a minimum free energy (mfe) < -20.0 kcal/mol, one G U mismatch was permitted in the seed sequence, and a maximum of three G U mismatches were allowed in the flanking sequence).
Similar(56)
A maximum of two mismatches was permitted for the purpose of alignment.
No mismatches were permitted.
At the alignment step, three mismatches were permitted.
Two mismatches were permitted in the 45-bp seed sequence.
Up to four mismatches were permitted to flag potential matches despite genotyping errors.
In the mapping process, the seed of a read was 30 bp, and 2 mismatches were permitted.
These conditions were achieved when a maximum of three mismatches were permitted (-v parameter) and when the best alignment regarding the number of mismatches was reported (--best parameter).
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CEO of Professional Science Editing for Scientists @ prosciediting.com