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The GT mismatch was created by replacing adenine with guanine maintaining a thymine on the complementary strand.
To achieve higher discrimination and specificity of the in-house developed AS-PCR primers, a mismatch was created three or four bases upstream from the 3′ end of the primers.
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The Packers prove that most mismatches are created simply by where certain players line up.
Mismatches are created, and the methyl-directed mismatch repair (MMR) system acts to eliminate the mismatches inhibiting recombination.
In Meyer et al. [28], the condition of mismatches is created and with behavioral experiment has reviewed the effects of such acoustic disturbances on speech perception in conditions approaching ecologically valid contexts.
To create oligonucleotides that bind both templates but induce a differential cleavage capacity between wild-type and mutant sequences, mismatches were created in the immediate adjacent nucleotides surrounding the mutation site of the binding oligonucleotide.
Mismatch distributions were created using ARLEQUIN version 2.000 [ 56] to detect historical demographic expansions for the major lineages (with or without range expansion in NCA analysis).
Expression vectors of Hox3-sg3 that contain mismatched nucleotides were created by inverse PCR using pSPU6>Hox3-sg3 as the template and PrimeSTAR thermostable DNA polymerase (Takara Bio).
A final set of incongruent audio-visual, Mismatch (MM), stimuli were created by mismatching auditory and visual tokens but maintaining the relative timing.
"The best thing about the spread for us was creating mismatches, finding it on the field and isolating a guy in space and picking on that linebacker covering the slot receiver," Couch said.
It is therefore imperative that more high-throughput and rapid approaches be adopted, such as next generation sequencing, tiling arrays or Surveyor™ nuclease technology, which utilizes a celery endonuclease highly specific for DNA mismatches that may be created following hybridization of patient and wild-type DNA (Pagniez-Mammeri et al., 2009).
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