As shown in Figure 3, and as for misclassified subtype 1a strains discussed above, misclassification of subtype 1b strains into subtype 1a was related to the presence of natural polymorphisms at positions 204 and 243.
As shown in Figure 2, misclassification of subtype 1a strains into subtype 1b in one or both assays was related to the presence of natural polymorphisms at nucleotide positions 204 and 243, both of which are located within the sequence of an INNO-LiPA HCV 1.0 probe.
The second correction factor adjusts the findings in order to avoid misclassification of subtype C infected individuals [ 13].
Another weakness is that misclassification probabilities of subtypes are likely to be independent of susceptibly loci and thus would tend to underestimate association strengths rather than creating spurious associations [ 36].
While depression may be influencing the number of patients reporting severe fatigue, especially among the relapsing-worsening MS subtype, it is also possible that some misclassification of disease subtype occurred between relapsing-worsening and primary progressive subtypes that contributed to more severe fatigue in the relapsing-worsening category.
Finally, misclassification of stroke subtype in those studies from countries with limited availability of medical imaging might have contributed to some of the observed heterogeneity.
Due to the possibility of misclassification between subtypes of hematological malignancies we excluded patients with prior history of any hematological malignancy from the study cohort.
The limitations of our study include a potential misclassification of preterm birth subtypes.
Thus, cautious interpretation of the expression of those markers seems crucial, as misinterpretation of results may lead to the misclassification of the molecular subtypes.
