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The majority of BESs had no detectable homology to known genes (49.5%) followed by sequences with similarity to known genes (44.3%), and miscellaneous sequences (6.2%) such as transposable element, retroelement, and organelle sequences.
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The two miscellaneous sequence features annotated were the control region and the light chain origin of replication.
There are 13 protein-coding genes, 22 tRNA and two rRNA genes along with two miscellaneous sequence features in the 16 724-bp genome.
Instead, the palindromic region was read as miscellaneous random sequences.
We also found some miscellaneous coding-sequences, which may or may not specify RR-related pepTable (Table 1).
Eleven sequences included in the 'Miscellaneous' category, according to Journet et al. [ 22] showed a different regulation in P665 compared to Messire.
Here we want to highlight five clusters with a total of 41 sequences found in the Miscellaneous class where no previous relationship within the families have been reported.
Sequences that did not match any proteins were annotated using the following Drosophila melanogaster release R5.1 sequences from FlyBase : microRNAs, miscellaneous RNAs, noncoding RNAs, all pseudogenes, all transposons and all transfer RNAs.
Miscellaneous nc RNAs are small noncoding RNAs sequences that do not carry information for producing proteins but can have various important functions in the cell.
Conversely "peripheral" functional categories such as signal transduction and gene regulation are overrepresented in that set, as well as IS-encoded transposases, conserved hypothetical proteins and sequences with ill-defined function (miscellaneous).
For example, in some cases, the use of Velvet-assembled contigs helped to extend sequence from contig ends, provided complementary strand data, and resolved miscellaneous ambiguities (thereby eliminating the need to generate additional sequence reads during the finishing process).
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CEO of Professional Science Editing for Scientists @ prosciediting.com