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NPC1 protein mirrored expression of mRNA in lean and obese individuals.
Previous studies with the mouse gene showed that intron 1 was required to promote good activity of a mPlp1-lacZ transgene in brain, which developmentally mirrored expression of the endogenous mPlp1 gene (Wight et al., 1993; Li et al., 2002).
Uptake rates of individual nitrogenous compounds closely mirrored expression of N-specific transporters during this study.
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P2b::CreBaC1, a BAC transgenic line expressing Cre recombinase from the Phox2b promoter in a pattern mirroring expression of the endogenous Phox2b gene (D'Autreaux et al., 2011 ).
Thus, RAD21 knock-down mirrors expression changes observed after depletion of pluripotency transcriptional regulators.
In conclusion, the simultaneous expression pattern of 160 genes in whole blood correlates with the severity of coronary artery disease and mirrors expression changes in the atherosclerotic vascular wall.
βKT activity, which overlaps with the EMC and PHB cycles, increased slightly initially then decreased to ∼60% of the original activity (Figure 7E), mirroring expression of phaA (Figure 6).
Previous studies have shown that reporter gene expression driven by components of the MMP-1 and MMP-13 promoters often do not mirror expression of the endogenous genes [ 20, 21].
Since reporter constructs driven by elements of the human MMP-1 and MMP-13 promoters often do not mirror expression of the endogenous genes, we shifted to a more direct, in vivo approach, using ChIP assays to detect changes in PPARγ binding at the DR-1/AP-1 site of the endogenous MMP-1 and MMP-13 promoters in genomic DNA.
When the transgene was positioned immediately downstream of the Evx2 poly(A)+ signal (relI), lacZ expression mirrored Evx2 expression; however, when the transgene was positioned half-way between the Evx2 and Hoxd13 initiation codons (relO), lacZ expression resembled Hoxd13 (i.e., lacked CNS expression in 11 dpc embryos) (Figure S1B) [14].
The PRLR expression pattern mirrored NHERF1 expression, except for the rare cases of lactation success in NHERF1 glands, suggesting the possibility of a link between PRLR and NHERF1.
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