Sentence examples for minutes with normal from inspiring English sources

Tissue sections were pre-incubated for 10 minutes with normal swine serum, followed by incubation for 1 hour with C4BP antibodies.

The nitrate myocardium content of hearts incubated for 45 minutes with normal and septic exosomes is demonstrated in Figure 6b.

After an initial washing step with PBS (Sigma, Munich, Germany), cells were blocked for 30 minutes with normal goat serum (Dako, Glostrup, Denmark) at a 1 10 dilution.

The sections were microwaved with an antigen retrieval solution (Target Retrieval, Dakocytomation, Carpinteria, CA, USA) and after blocking of endogenous peroxidase activity with 3% hydrogen peroxide, the sections were further blocked for 30 minutes with normal rabbit serum.

Antro-duodenal manometry was considered normal if i) phase 3 of the MMC, as defined by repetitive antral contractions occurring at a rate of 2 3 per minute and 10 12 per minute small bowel contractions, lasted for more than 2 minutes with normal antegrade propagation ii) there was normal stomach antrum and small bowel response to a meal [ 25].

Sections were fixed in acetone for 10 minutes and with normal horse serum (VECTASTAIN® Elite ABC kit) for 15 minutes.

Cells were grown in 8-well chamber slides, fixed with ice-cold methanol for 2 minutes, blocked with normal serum, and incubated with primary antibodies for 1 hour at room temperature.

Sections were washed with PBS (pH 7.4) and incubated for 30 minutes with 1.5% normal goat serum (Santa Cruz Biotechnologies Inc., SC, CA, USA) to block nonspecific binding.

For paraffin-embedded sections obtained from quadriceps muscle biopsies, after paraffine removal, sections were rehydrated and incubated for 30 minutes with 10% normal goat serum and processed for viral antigens detection.

Cultures were fixed in acetone for 10 min. Immunocytochemistry was performed after incubation for 30 minutes with 10% normal goat serum diluted in PBS, to avoid nonspecific antibody binding.

Slides were then incubated for 20 minutes with diluted normal blocking serum.