Sentence examples for minutes with continuous from inspiring English sources

In line with other studies, we distinguished sedentary behaviour from time when the monitor was not worn by excluding periods of >10 minutes with continuous zeros [25].

The test tubes were then incubated on ice for 30 minutes with continuous shaking, protected from light.

At 2 hours, a subset of plates were treated with OxPAPC (20 μg/mL) for 15 minutes followed by treatment with thrombin (0.5 U/mL) and incubation for 15, 30, or 50 minutes with continuous exposure to CS.

A 2 : 1 stoichiometric mixture of CBZ (4.725 g) and NIC (1.181 g) was placed in mortar, crushed, and ground vigorously for 30 minutes with continuous dilutions with methanol [ 26].

For culturing, primary tumors were chopped into small pieces, washed in PBS and dissociated in PBS with collagenase IV (final concentration of 6 mg/ml, C5138; Sigma-Aldrich, St Louis, MO, USA), hyaluronidase (final concentration of 1 mg/ml, H3506; Sigma-Aldrich) and DNase I (final concentration of 0.25 mg/ml, D5025-15KU; Sigma-Aldrich) for 30 minutes with continuous agitation at 37°C.

Co ., suspended in 5 ml of enzymatic solution [2.5 mg/ml trypsin (Life Technologies Inc ., 5 mg/ml albumin (Life Technologies Inc ., and 850 U/ml collagenase type II Life Technologies Incc). in phosphate buffered saline], and incubated at 37°C for 20 minutes with continuous stirring.

The method is based on the treatment of the culture with trypsin for around 1 minute, with continuous microscopic observation.

The cultures were then placed in an airtight incubation chamber (CBS Scientific) equipped with inlet and outlet valves, and flushed for 4 minutes with a continuous influx of 1% O2, 5% CO2, 94% N2 gas at a flow rate of 20 L/minute.

The minced tissue was dropped in the solution, weighed, and subsequently incubated for 15 minutes at 37°C with continuous rotation at 16 to 20 revolutions/minute (rpm).

The standards, samples or blanks were mixed with beads in a final volume of 100 μl and incubated for 30 minutes at 25°C with continuous shaking.

The remaining study used 5 minutes of ischemia with continuous reperfusion up to the time of portal triad clamping (Table 2).