Sentence examples for minutes with buffer from inspiring English sources

The hearts were perfused for another 5 minutes with buffer alone and then fixed under pressure for 10 minutes with 4% paraformaldehyde (pH 7.4) (PFA).

The column bound material was recovered by elution for 8 minutes with buffer B at 1 ml/minute.

Peptide samples were injected onto a Pepmap100 μ-guard column (Dionex, Sunnyvale, CA) via a Famos Autosampler (Dionex) and washed for 10 minutes with Buffer A (2% Acetonitrile, 0.1% Formic Acid) at 15 μL/min.

After each analysis, the column was cleaned with 100% Buffer B and then equilibrated for 2 minutes with Buffers A : B 54 : 56 (MT1 and MT3) 5258 58 (MT2).

After a 30-minute labeling incubation, the reaction is split and incubated for an additional 5-minutes with buffer or Npl3.

Samples were washed twice (15 minutes and 60 minutes) with McIlvaines buffer, and mounted in McIlvaines buffer with 50% glycerol.

Cells were centrifuged at 4000 rpm for 5 minutes, washed with buffer A [50 mM Tris/HCl, 1 mM EDTA, pH 7.5] and disintegrated by vortexing with zircon beads (diameter 0.7 μm, Biospec, Bartlesville, OK, USA) 2 1 in buffer A with plant protease inhibitors (Sigma-Aldrich, Prague, Czech Republic) for 6 minutes.

After binding, beads were washed three times for 5 10 minutes with wash buffer prior to addition of sample buffer.

The membranes were washed twice for 5 minutes with TBS buffer [20 mM Tris HCl (pH 7.6), 137 mM NaCl], blocked for 1 hour with 5% BSA in TBS buffer, incubated overnight at 4°C with affinity column purified rabbit GvpC antibodies (Thermo Scientific) diluted 1 500 [ 15] or rabbit anti-His-tag antibody (Cell Signaling Technology, Beverly, MA) diluted 1 750.

To conclude the experiment the chamber was rinsed for 20 minutes with PBS buffer at 10 μl/min.

Nonspecific staining was blocked (30 minutes) with blocking buffer (10% normal donkey serum, 1% bovine serum albumin [BSA] in PBS).