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Reverse transcriptase reaction was performed by incubating for 10 minutes at room temp, 50 minutes at 42°C, then 95°C for 5 minutes to terminate the reaction.
Subsequently, the temperature was increased to 70°C for 15 minutes to terminate the reaction.
After one hour the temperature was increased to 70°C for 15 minutes to terminate the reaction.
After 1 hour the temperature was increased to 70°C for 15 minutes to terminate the reaction.
The samples were incubated at different temperatures starting at 25°C for 10 minutes, followed by 42°C for 60 minutes and finally 70°C for 15 minutes to terminate the reaction.
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Samples were subsequently heated for 15 minutes at 70°C to terminate the reverse transcription reaction.
Dynegy Chairman and Chief Executive Chuck Watson said last-minute disclosures led him to terminate an agreement to acquire Enron and keep it afloat.
In the more superficial parts of the dermis, minute lymph vessels that appear to terminate in blind sacs function as affluents of a superficial lymphatic net that in turn opens into vessels that become progressively larger in the deeper portions of the dermis.
After 5 minutes, 100 μL of 1 N HCI was added to terminate the reaction.
Sad to terminate.
tries to terminate the deal.
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