Sentence examples for minutes the sample from inspiring English sources
From 0 15 minutes the sample is loaded on to the column at 500 nl/min in 100% Buffer A (97% water, 2.5% acetonitrile, 0.5% acetic acid).
After annealing at 60°C for 15 minutes, the sample was kept on ice for one minute.
After incubation below the Triton X-114 cloud point on ice for 15 minutes, the sample was heated to 37°C for 10 minutes to allow the two phases to separate, followed by centrifugation at 400 x g for 2 minutes at room temperature.
After 20 minutes the sample was mixed.
After sonication for 20 minutes, the sample was filtered through a 0.45-μm nylon membrane and injected into the HPLC.
They are rapid, can be carried out in just a few minutes, the sample preparation is often very short and they do not need equipment.
After two minutes the sampling rate is decreased to 0.1 Hz, for the flow dynamic next slows down considerably.
Despite its running time of 22 minutes, the sample-laden single reached #78 on the British singles charts.
After the application of DCS, pro-inflammatory agent or combination of both for 45 minutes, the samples were further incubated for 75 minutes.
After thirty minutes, the samples were boiled in sample loading buffer and separated by SDS-PAGE, with subsequent visualization of phosphorylation by autoradiography.
After drying the slides for 20 minutes, the samples were stained with 0.4% Toluidine Blue O solution (Sigma-Aldrich) and washed with phosphate buffer saline (PBS).
