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After permeabilization with PBS containing 0.1% Triton X-100 for 10 minutes sections were blocked for 1 hour with PBT (0.15% BSA, 0.1% Tween 20 in PBS).
After pretreated with 0.3% hydrogen peroxide in PBS for 15 minutes, sections were preincubated for 1 hour in blocking solution (0.1 M phosphate buffer, 0.25% Triton X-100, and 5% normal goat serum).
After leaving at room temperature for 20 minutes, sections were immersed in phosphate-buffered saline (PBS).
After cooling at room temperature for 20 minutes, sections were rinsed in deionized water.
After incubation of each antibody for 30 minutes, sections were rinsed and incubated with the appropriate secondary antibodies (DAKO).
After deparaffinization in xylene for 30 minutes, sections were rehydrated through a gradient with decreasing proportions of ethanol.
Similar(33)
Four weeks after transplantation, hearts were arrested with 15% KCl, perfused with formaldehyde 4% (15 mmHg) for 20 minutes, sectioned into 3 4 transverse slices, and parallel to the atrioventricular ring.
Provide the ability to set the minutes section of the counter.
If they are smaller, you will have to subtract 1 hour from the hour section and add 60 minutes to the minutes section.
Specify how often you want Word to save your document and program state by clicking on the arrows in the minutes section.
The students divided the May corpus into twenty-minute sections.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com