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Total imaging time is approximately 4 minutes per sequence or 12 minutes for actual imaging (this relatively long time was not inconsistent with protocols during the time of the study).
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GRISOTTO- was able to use all of them taking only 1-4 minutes, per sequence-set, to report a motif.
The whole dataset was processed in approximately 19.5 days, using 50 of the 1,000 nodes in our IBM e1350 cluster, powered by two 2.0 GHz dual core AMD Opteron 270 processors per node, i.e. the average running time of EFICAz in a single 2.0 GHz core was 3.24 minutes per genomic sequence.
Besides effectiveness, GRISOTTO also showed to be very efficient, taking around 2 to 3 seconds per yeast sequence-set, that have around 200 sequences of 500 bp, and 1 to 4 minutes per mouse sequence-set, that have from around 1000 to 40000 sequences of 200 bp.
The new systems require just 15 minutes of manual setup time per sequencing run and less than 45 minutes of hands-on work from DNA to data when using the Ion Chef System for automated Ion AmpliSeq library construction, template preparation, and chip loading.
I recommend building a curve per sequence.
How many minutes per visit?
20 minutes per bauble.
Approximately 12 minutes per group.
Activities measured in minutes per day.
BBC Productions, Wales - 10x15 minutes per year.
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