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We did the exchange, which took about fifteen minutes of loading luggage from one car to the other, his mom hitting the loo, and saying good-bye.
Following 5 minutes of loading and washing in mobile phase A, peptides were eluted using a linear gradient of 0% to 50% B over 45 min, followed by a linear gradient of 50% to 100% B over 20 min. One-minute fractions were collected and dried by vacuum centrifugation and then redissolved by shaking in 100 µl of 5% formic acid.
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The test will be terminated after 20 minutes of loaded cycling if volitional exhaustion is not reached.
Customers pay for Lugg at a base price of $35, plus $2 per mile and $1 per minute of loading and unloading.
"It took a couple of minutes to load the purchase app," said Eboni Jones, who does marketing and communications for Parish Entertainment Group, a beta partner of the service.
Wait for at least a couple of minutes after they sign in before initiating a conversation, especially if this person has a slow computer, because it may take a couple of minutes to load.
But that quality comes at a price: different sections of the app took minutes to load for the first time, even on a high-speed Wi-Fi connection.
In 2-SNP analyses, assuming a dominant genetic model, we observed a significant interaction between rs3790261 in SLC24A3 and rs11893826 in SLC8A1 affecting absolute changes in SBP and Na+ excretion in response to 120 minutes of salt loading (PNat120) (p interaction = 1E-03, p model = 9.12E-05) (Figure 2).
Moreover, c-Fos and c-Jun, the downstream of ERK 1/2 and JNK, were examined after 15 minutes of mechanical loading.
Our previous work shows that 27-HC is synthesized within minutes of cholesterol loading, indicating their ability to rapidly respond to changes and mediate sterol-homeostatic responses.
Others took five minutes to load.
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