The critical time identified was from 65 to 100 minutes of development, during which nuclear division cycles are very rapid and may only suppsort the synthesis of short zygotic transcripts [33].
In the experiments described below, embryos were allowed to develop in a temperature step with their anterior half at 27°C and their posterior half at 20°C for the first 200 minutes of development, until the cellular blastoderm stage.
ago2414 embryos developed normally under uniform temperature conditions (Figure 4A, C), but they displayed abnormal Eve expression when developing in a temperature step with the anterior half at 27°C and the posterior half at 20°C for the first 200 minutes of development (Figure 4B, D).
To test our hypothesis, WT embryos were exposed to a larger temperature step, with their anterior half at 27°C and their posterior half at 17°C for the first 200 minutes of development, and then allowed to reach larval stage at uniform 24°C.
At uniform 23°C in a microfluidic device, cytoplasmic movement was minimal in wild-type embryos between 65 and 95 minutes of development (Figure 1A, Movie S1, top panel), as shown by particle tracers both at the surface (Figure 1A, black dots) and within the cytoplasm (Figure 1A, blue, red, and green dots).
A 3-hour upshift after 680 minutes of development produced no supernumerary IPC NBs; hence, the end of the competence period for pPIm cells to take the IPC NB fate occurs at 500 minutes of development, corresponding to the end of stage 12, when the IPC NB first expresses Dac.
O'Donnell's approach to writing music for games is to put in the audio at the last minute of development, so that his music meshes with the game play in the best possible way; he still had not added the score when Halo 3 was demoed at Electronic Entertainment Expo 2007, less than three months away from the game's debut.
Mr. O'Neal, who was appointed by Mr. Bloomberg in April, has missed eight of the 11 board meetings since he was appointed, according to a review of the minutes of the development corporation's board meetings.
The data consist of 9 time points spanning 71 minutes of Drosophila development, and at each time point maximally 53 nuclei with data points for the four gap genes, and the four external inputs.
One hundred microliters of paranitrophenylphosphate (1 mg/mL) diluted in substrate buffer (carbonate-bicarbonate buffer with 10% diethanolamine and 3 mM MgCl2, pH 9.6) was added as a substrate and the absorbance of the yellow product was measured at 405 nm using a Spectramax 190 ELISA plate reader (Molecular Devices, Sunnyvale, CA) after 20 minutes of color development at 25°C in the dark.
"We've lost nearly six minutes of important plot development; the audience won't have a clue what's happening".
