Sentence examples for minutes of centrifugation from inspiring English sources

After 20 minutes of centrifugation at 4°C and 13.000 rpm, the precipitated DNA was washed with 70% ethanol, re-precipitated by 5 minutes of centrifugation at 4°C and 13.000 rpm.

After 30 minutes of centrifugation in a swinging bucket rotor at 1400 rpm, the upper layer was aspirated and the mononuclear cell layer was collected.

Cellular debris was removed by 5 minutes of centrifugation (6000 g at 4°C) and the supernatant was flash-frozen at −80°C.

Protein supernatant was extracted after 20 minutes of centrifugation at 3000 rpm at 4°C, and total protein concentration determined via Bradford assay.

After 15 minutes of centrifugation, an aliquot of the lysates was removed for Western blotting, and the remainder was immunoprecipitated overnight with 1.5 µg anti-C/EBPα, anti-HA, anti-STAT3, and anti-IRS1, and 40 µl of protein G or A-Sepharose (50% suspension).

After 10 minutes of centrifugation at 15,000 g, supernatant is reduced in Laemmli buffer and heated at 90°C for 6 min. Protein extract were subjected to electrophoresis on a 10% acrylamid reducing gel, and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Millipore).

Homogenates were enriched for ECM by two cycles of centrifugation (relative centrifugal force (RCF max 110,000 × g, 30 minutes, 4°C).

The SF samples were pretreated with 5 mg/ml hyaluronidase (Sigma-Aldrich, St . Louis MO, US) dissolved in phosphate buffered saline for 5 minutes and clarified by means of centrifugation at 5,000 g for 10 minutes.

An optimal centrifugation time of 20 minutes and centrifugation container volume of 50 mL at 70 80% full of FS for conditioned FS were obtained.

To prevent possible damage to patches, the methanol was evaporated with a speed vacuum for 20 minutes following centrifugation of the 96 well plates at 1000×rpm for 20 minutes.

It has been reported that a shorter time of centrifugation, three minutes only, may result in a higher sensitivity for T. vivax.