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To quantify receptor-Rluc expression luminescence readings were performed after 10 minutes of adding 5 µM coelenterazine H.
The tubes were immediately placed on ice, then frozen in a −80°C freezer within 10 minutes of adding the cells.
Clearly the mechanism by which PPAR agonists dilate vessels acutely must be mediated independently of gene induction as the response is seen within minutes of adding the drug.
All optical densities (ODs) were read at 450 nm within 30 minutes of adding stop solution.
An absorption ratio of 540 nm/595 nm was determined within 5 to 10 minutes of adding DMB solution.
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