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Ronaldo's acrobatic attempt at a fourth was blocked by Eboue, who had initially allowed the Portuguese space for the shot, but Mourinho's men had long done their job, and played out the remaining minutes in control to set up what looks like a straightforward second leg.
Cells treated with 200 Gray of ionizing radiation septated 40 minutes later than untreated cells (120 minutes and 160 minutes in control and treated sample, respectively).
The average time spent in mitosis was 6 hours 35 minutes (s.d. = 3 hours 56 minutes; n = 26), compared with 69 minutes in control cells (s.d. = 24 minutes; n = 36).
Following 0.25 mM treatment with diamide, approximately 80% of reduced glutathione (GSH) was oxidized within 5 minutes and the pre-treatment levels were restored within 45 minutes in control RBCs.
Neurons were recorded for 10 minutes in control and for at least one hour after the cellular conditioning protocol; long-term synaptic efficacy changes were measured around one hour.
Synaptic recordings were made for 10 to 25 minutes in control conditions following 10 to 20 minutes of 100 μMSNAP-5114 application and 10 to 30 minutes washout.
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In these cells, metaphase lasted 1 6× longer than in control cells (on average 110 minutes with s.d. = 78 minutes, compared with 44 minutes with s.d. = 25 minutes in controls).
EMS CPR was initiated at 5 minutes in RC versus 7 minutes in controls.
EMS was initiated at 7 minutes in RC versus 9 minutes in controls.
Despite this, the frequency of SPWs was reduced by more than 80% in slices lacking mossy fibers, as is evident from a comparison of the number of waves per minute in control and experimental slices (Fig. 4D,E).
Cell cultures were set in triplicates, and results are presented as proliferation index (median of count per minute in wells with conA minus the median of count per minute in control wells).
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