Sentence examples for minutes in buffer from inspiring English sources

The 3Cpro, the best fluorogenic peptide and compound incubate a few minutes in buffer.

The loading procedure was performed twice before the vessels were washed and equilibrated for 15 minutes in buffer solution.

For reactions with D-loops containing a biotinylated nucleotide the substrate was pre-incubated at 37°C for 10 minutes in buffer alone or with 1.5 nM streptavidin prior to WRN addition.

Ferricyanide reductase assays were conducted at room temperature and the absorbance monitored at 410 nm for 2 minutes in buffer containing 10 mM potassium phosphate (pH 7.0), 1 mM EDTA, 1 mM K3FeCN6, and 10 mM KCN [46].

Cells were stained at 4°C for 30 minutes in buffer (2% fetal calf serum/0.05% sodium azide/PBS) containing antibodies, washed, and resuspended in 1% paraformaldehyde/PBS. Antibodies used included: CD3-phycoerythrin (PE), CD4-fluorescein isothiocyanate (FITC), CD8-peridinin chlorophyll protein (PerCP), CD56-allophycocyanin (APC), CD19-FITC, CD14-PerCP-Cyanine5.5.

The slides were then left to stand for 10 minutes in buffer at room temperature before being washed thoroughly in tap water.

In brief, tissue sections were subjected to pre-wash twice for 5 minutes in buffers, followed by incubating with [3H]gaboxadol: 10–400 nM and post-wash with buffers for 4 quick dips.

Subsequently, the cells were lysed in a Dounce homogenizer (Fisher) for 2 min (75 strokes per minute) in lysis buffer.

Samples were incubated at 4°C for several days and rinsed three times for 15-minutes in buffer and then post-fixed in 2% osmium tetroxide in 0.1 M sodium cacodylate buffer, pH = 6.8 for 2 hours at 4°C.

Briefly, after treatment, cells were washed three times in Buffer A (20 mM Tris-HCl, pH 7.4, 137 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, and 0.1 mM sodium orthovanadate) and incubated at 4-degrees C for 30-minutes in Buffer A plus 1% NP-40 and 1 mM PMSF.

The reactions were terminated by washing 2 minutes in wash buffer 1 and 2 minutes in wash buffer 2. The rolling circle products were detected by hybridizing fluorescently labeled oligonucleotides in a buffer containing 20% formamide, 2× SSC, 5% glycerol and 0.17 μM of either ID 16 or anti ID 16 for 30 min at 37°C.