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Data is sent to company servers - the cloud - every 10 minutes, for analysis and to help with grid and supply balancing.
During the first hour of reaction, 2 ml sample volumes were collected from the reactor every 10-20 minutes for analysis of solution composition; in the longer experiments, samples were collected at 30 minute intervals during the second hour.
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Data were selected for three distinct epochs (minutes 2 4; minutes 15 17; and minutes 27 29) for analysis to represent behaviour over the full 30-minute test interval.
Data were recorded in a continuous mode (sampling rate 312.5 Hertz [Hz]) starting with eyes open and closed (counterbalanced over sessions and subjects) for 1.5 minutes each for analysis of spontaneous cortical activity, followed by the auditory mismatch experiment.
Given the evidence provided, the EPHect SOPs advise, where LN2 or −80°C freezers are available, to snap freeze tissues as soon as possible after collection (within 15 minutes for RNA analysis) or otherwise immerse in RNA-stabilizing solution, followed by freezing if molecular analysis is to be conducted in the future.
Therefore, in order to capture the effects of the highly ephemeral cicadas resource, mouse plasma tissues were separated from whole blood by centrifugation (at 1,200 g for 10 minutes) for isotope analysis for the cicadas grids.
One milliliter samples were removed every 10-15 minutes for HPLC analysis.
Aliquots were removed after 60, 90 and 120 minutes for FACS analysis and 2μ plasmid isolation.
Samples were collected as frequently as every 15 minutes for trend analysis.
We video-recorded the behavior of the fish for six minutes for later analysis using an animal tracking software package (TopScan Lite, Reston, Virginia, USA).
However, rhythm analysis during CPR is conceived to continuously diagnose the rhythm with the objective of improving CPR delivery compared to the standard CPR protocol, which requires interrupting CPR every two minutes for rhythm analysis.
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