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After centrifugation at 16,000 g for 30 minutes, extracts were diluted with 9 volumes of buffer A containing 1% Triton, and then immunoprecipitated with FLAG M2-agarose (Sigma).
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Finally, we note that the DBLModeller model extraction times do not exceed 3 minutes: extracting the model for the largest of our schemas took only 174s.
In the first run, attaching and lowering the water sampling device took 44 minutes; extracting it took 32 minutes, and the team was reasonably satisfied.
To quantify this, we recorded continuously for 5 minutes, extracted the spike timings, and calculated the instantaneous firing frequency and coefficient of variation (CV) from the ISI.
The LPS groups received an intravenous LPS injection (1.6 mg/kg during 10 minutes) (extracted from Escherichia coli, serotype 0127 B8; Sigma-Aldrich).
After two hours at room temperature, clotted blood samples were centrifuged at 1,000 x g for 15 minutes, extracted serum were stored at −80°C until subsequent analysis.
After incubation for 24 h, cells were washed once with 2 ml of ice-cold PBS for 10 minutes, extracted three times with 2 ml of cold 10% TCA for 5 minutes each time, and solubilized for at least 30 minutes at room temperature in 0.2 ml 0.3 N NaOH, 1% SDS.
Johnson et al.'s (1959) eight different dysfluency signs were coded on each speech sample and counts of dysfluency signs 2 3 and 4 6 on two-minute extracts were made, participant by participant.
First, the show in question, of which I saw only a 40-minute extract.
One was the fact that BBC Television showed an 18-minute extract which led to the box-office being besieged.
Three finalists will have a 15-minute extract performed by major actors at a live public event later this year, when a panel of key industry figures will make their final decision.
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