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After a brief interval, the mouse was placed back into the chamber for another 5 minutes; exploration was assessed by analyzing the number of contacts, defined as nose-first approaches to within 2 4 cm of an object, with a given object in a trial, and habituation was assessed by comparing the number of contacts made with each object over the course of two subsequent trials, three in all.
Mice were placed in the center for 10 minutes exploration of all arms.
On completion of 10 minutes exploration, the mouse was returned to its cage for a 3-h delay.
Twenty-four hours later, on the third and last day, the animals were placed in the same context for 5 minutes exploration (contextual fear assessment).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com