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Make a movie in minutes by adding video, photos, music and sound effects.
The dye was resuspended for 30 minutes by adding 100 ml of ethanol 100% into each well.
The reaction was stopped in 10 minutes by adding an equal volume of 10%Na2CO3O3.
The conjugate substrate reaction was terminated after 20 minutes by adding a stop solution.
The reaction was stopped after 20 minutes by adding 25 ml of 3 N sulfuric acid.
In A. nidulans it was observed that a low steady-state level of creA mRNA can be increased within minutes by adding a repressing carbon source.
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If your first timed run lasted under 30 minutes, start by adding 5 minutes next time.
Sections were preincubated subsequently with 0.05% DAB in 0.1 M PB for 5 minutes, incubated by adding 0.01% hydrogen peroxide to the same solution for 5 minutes and washed in 0.1 M PB for 2 hours at RT. Stained sections were osmicated (1% OsO4 in 0.1 M PB, pH 7.4, 20 minutes), dehydrated in graded alcohols to propylene oxide and plastic-embedded flat in Epon 812.
Cell membrane integrity was then monitored at 10 minutes intervals by adding propidium iodide to the treated parasites.
Cell membrane integrity was monitored at 10 minutes intervals by adding propidium iodide to the treated parasite samples (Figure 2).
The reaction was stopped 15 minutes later by adding 50 μL/well H2SO4 solution.
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CEO of Professional Science Editing for Scientists @ prosciediting.com