Sentence examples for minutes both at from inspiring English sources
Kurt Thomas, Rasheed Wallace and Marcus Camby are expected to share minutes both at the forward and center positions coming off the bench.
Sections were incubated with primary antibodies for 2 hours, HRP-conjugated secondary antibodies for 30 minutes, both at room temperature.
Sections were incubated with primary antibodies for 2 hours, FITC and TxR-conjugated secondary antibodies for 30 minutes, both at room temperature.
The primary antibody (anti His-tag or mAb VAP-5) was incubated for 1 hour and the secondary antibody for 45 minutes, both at room temperature.
Sections were then incubated with a peroxidaxe blocking reagent® (Dako, Glostrup, Denmark) for 10 minutes and with 0.5% Casein (Sigma-Aldrich, Denmark) in Tris-buffered saline (TBS) for 20 minutes, both at room temperature.
To detect NCBP-2 protein, dissected gonads were fixed in aldehyde fixative (3.5% formaldehyde, 0.25% glutaraldehyde, 0.1 M potassium phosphate, pH 7.2) for 15 minutes, followed by incubation in methanol for 5 minutes and acetone for 10 minutes, both at -20°C.
We observe a stiffening of cells submitted to a constant force within a few minutes, coupled to actin recruitment both at the bead-cell contact and up to several micrometers from the stress application zone.
At 180 minutes, both treatments had more than doubled urinary excretion and the ratio between excreted and infused fluid volumes (Kruskal-Wallis test, P < 0.01).
In addition, patients exhibited at least 8/66 swollen joints, at least 10/68 painful joints and at least one of the following three conditions: erythrocyte sedimentation rate of at least 28 mm/hour, C-reactive protein (CRP) of at least 15 mg/litre or morning stiffness for at least 45 minutes at both screening and baseline time points.
"They executed well for 40 minutes at both ends of the floor.
"They're huge up front, and they've got the two guards and they just attack you for 40 minutes at both ends of the floor," Jarvis said Saturday after the Red Storm's 86-74 victory at West Virginia.
