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Interventions: Randomized infusions of diltiazem (bolus 0.25 mg/kg followed by a continuous infusion of 1.7 μg/kg/min) (DTZ, n = 12) or placebo (C, n = 12) were started 30 minutes before induction of anesthesia and continued for 24 hours.
Twenty minutes before induction of thrombosis (TI), animals received either a single IV-bolus of AT (250 IU/kg bw; Kybernin HS, CSL Behring, Marburg, Germany; n = 16), or saline (control; n = 16) corresponding to the fluid amount applied in the verum experiments (Fig. 1, Table 1).
To compare SOD3 to clinically approved medication we gave an intra-peritoneal injection of dexamethasone (Oradexon) to animals 30 minutes before induction of peritoneal inflammation.
All patients received midazolam 0.1 mg/kg orally 30 minutes before induction of anaesthesia.
Premedication consisted of oral midazolam 7.5 mg given on the ward 20 to 30 minutes before induction of anesthesia.
All patients were pre-medicated with midazolam 0.1 mg·kg-1 orally 30 minutes before induction of anaesthesia.
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Stretch for ten minutes before jogging.
In such groups the animals were simultaneously treated with EDTA and L-NAME 30 minutes before the induction of Isc (group 5) and 30 minutes before the induction of Isc/R (group 6).
Intravenous cefazoline (2 g) was also administered to all patients 30 minutes before anaesthetic induction.
As premedication, oral midazolam (0.15 mg/kgBW) was administered 30 minutes before the induction of anaesthesia.
Treatment with EDTA was performed 30 minutes before the induction of ischemia.
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