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MD-Ms (1 × 10) were incubated with 20 μg zymosan particles for 20 minutes at either 37 or 4°C.
Cells were preincubated for 15 minutes at either 4°C or 37°C, followed by incubation for 1 hour with FD (1 mg/ml).
Again, no between-group difference in change was apparent in the weekly minutes at either of the follow-up (GMR 1.22, 95%CI 0.96 to 1.54; GMR 1.09, 95%CI 0.85 to 1.39).
Female mice were subsequently inoculated with H-TdR (50 μCi, 100 μl) and euthanized within 90 minutes at either 4 to 6, 8 to 10, or 12 to 15 days after coitus.
Following the mixings of DNA and the dyes, the resulting solutions were incubated for 30 minutes at either room temperature or 50°C before measurements were taken at the same respective incubation temperature.
Cells were rinsed thoroughly with Kreb's phosphate buffer (KB) and incubated in medium containing either 40 nM [H]DA or 40 nM [H]DA and 4 μM unlabeled 'cold' DA (Perkin-Elmer, Sigma-Aldrich) and incubated for 5 minutes at either 37°C or 4°C.
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It was settled by an own-goal and contained 10 minutes of excitement at either end.
Eighteen children with autism, ages 3 16 years, underwent 40 hyperbaric sessions of 45 minutes duration each at either 1.5 atmospheres (atm) and 100% oxygen, or at 1.3 atm and 24% oxygen.
The cultures were supplemented with CaCl2 (to 5 mM) and infected for 20 minutes at 30°C with either 50 µl of P1vir (2.5×106 pfu) or spent LB (AB1157 grown to saturation with the cells removed by filtration).
Lysates were re-suspended in 1X Laemmli buffer (BioRad) containing beta-Mercaptoethanol, boiled for 5 minutes at 1000C, and either run on precast 4-20% Gels or 10% Criterion gels from BioRad.
Although HRR was significantly different between sexes, after controlling for VO2 peak, there were no differences between males and females at either minute one or minute two following maximal exercise.
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