Sentence examples for minutes are maintained from inspiring English sources

The amplification was associated with extra-chromosomal double minutes; these are unstable genomic elements that were detected in vivo by fluorescence in situ hybridization analysis of mouse-derived tumors but were rapidly lost in primary cultures, probably because double minutes are maintained only in the presence of in vivo selection pressures within the breast microenvironment.

Initial conditions (98% A) then a linear gradient to 100% B over 15 minutes, was maintained for 10 minutes before returning to the initial gradient conditions.

The biggest challenge, said Ms. Onufer, who won the women's division in about 34 minutes, was maintaining an even pace so she wouldn't tire too quickly.

Some members of this protein family have extremely short half-lives, in the order of 5 minutes [ 98], and are maintained at steady state level in cells with a particular auxin concentration.

The compounds were eluted with a gradient elution of mobile phase A (100% acetronitrile) and B (1% acetic acid in deionized water) where A increased from 3%to8%8% in 5 minutes, to 10% by 25 minutes and was maintained at 10% for 20 minutes, then returned to initial condition (3%) in 10 minutes and remained for 5 minutes before next injection.

The mobile phase began at 25% B with a linear gradient to 95% B at 7 minutes, which was maintained for 1.5 minutes, followed by re-equilibration to 25% B. Separation was achieved with a solid-core C18 column (KinetexXB -C18, 100 mm × 2.1 mm, 2.6 μm particles, Phenomenex, Torrance, CA, USA) maintained at 40°C.

The mobile phase began at 10% B with a linear gradient to 95% B at 7 minutes, which was maintained for 2 minutes, followed by re-equilibration to 10% B. Separation was achieved with an ACE3 columnlumn (150 mm × 2.1 mm, 3 μm particles, Advanced Chromatography Technologies, LTD MacModd, Chadds Ford, PA, USA) maintained at 40°C.

The mobile phase began at 15% B with a linear gradient to 95% B at 7 minutes, which was maintained for 1.5 minutes, followed by a re-equilibration to 15% B. Separation was achieved with a solid-core c18 column (KinetexXB -C18, 100 mm × 2.1 mm, 2.6 μm particles, Phenomenex, Torrance, CA, USA) maintained at 40°C.

For the first 15 minutes, ACN was maintained at 20% and then it was gradually raised to 80% up to 40 minutes, further brought to 100% till 50 minutes, and thereafter maintained for 15 minutes.

Mice were anesthetized at 2% isoflurane with 1 L/hr oxygen during induction for 20 minutes and were maintained at 1% to 1.5% isoflurane during imaging.

A continuous flow of 200 sccm (standard cubic centimeters per minute) Ar gas was maintained during the entire process.