Sentence examples for minutes and samples from inspiring English sources

The plate was shaken for 3 minutes and samples were transferred to a white 96-well plate.

The reaction was terminated at 85°C for 5 minutes and samples were diluted 1 10.

Amplification was followed by a final elongation step at 72°C for 5 minutes and samples held at 4°C.

The reverse transcription was conducted at 50°C for 50 minutes and 85°C for five minutes, and samples were stored at -20°C until use.

The enzymatic reaction was stopped by incubation at 95°C for 10 minutes and samples were centrifuged at 10,000 g for 5 seconds.

Phalloidin (Sigma) was then added at 1 1000 for 20 minutes and samples mounted in Vectashield (Vector) with 2 μg/ml DAPI.

For melting experiments, temperature increments were either 1 or 2 °C per minute, and samples were allowed to equilibrate for 30 s before collecting each spectrum.

Collection was completed in 20 minutes [23] and samples were stored at −80°C until analyzed.

The blood sample was centrifuged within a few minutes and plasma samples were transferred and frozen to be later assayed for cortisol concentration (CORT) by radioimmunoassay [ 49].

Retina samples were centrifuged at 70,000 rpm for 20 minutes and blood samples were centrifuged at 12,000 rpm for 15 minutes.

Blood samples were kept at room temperature for 30 minutes, and plasma samples were separated and were stored at −70C°.