Sentence examples for minutes and resolved from inspiring English sources

Equal numbers of whole ganglia were harvested and lysed at 4°C for 40 minutes and resolved by SDS-PAGE.

Samples were prepared for SDS-PAGE by the addition of 4 volumes of 4X Laemmli's sample buffer, boiled for 3 minutes and resolved on 12% SDS-PAGE gels.

10 µg of protein was boiled in Laemmli sample buffer for 10 minutes and resolved on 4 to 15% sodium dodecyl sulfate-polyacrylamide gel.

Samples were prepared for SDS-PAGE by the addition of 10 volumes of 2X Laemmli's sample buffer [31], boiled for 3 minutes and resolved on 12% SDS-PAGE gels.

In brief, to analyze MPER presentation on selected vectors, 1010 viral particle (VP) were boiled in Laemmli sample buffer for 10 minutes and resolved on 4 to 15% sodium dodecyl sulfate-polyacrylamide gel.

Finally the beads were resuspended in 2xSDS-sample buffer (120 mM Tris/HCl, 10% SDS, 20% Glycerol, 20% 2-mercaptoethanol, bromphenol blue, pH 6.8), heated to 95°C for 15 minutes and resolved by SDS-PAGE.

Individuals have benefited from the viral virtues of social media: Musician Dave Carroll got the attention of United Airlines when his YouTube video about how the airline broke his guitar reached millions of viewers; TechCrunch founder Michael Arrington got Comcast to respond to him in 20 minutes and resolve a connection issue he had complained about to his 12,000 Twitter followers.

Boiled samples were centrifuged at 1700 g for 1 minute at 4°C, and resolved on 12% SDS-PAGE for 2.5 hours at 150 V.

Protein extracts (10 μg) were centrifuged at 4°C for 2 minutes at 14,000 g and resolved on NuPAGE 4-12% Bis-Tris Gels (Invitrogen Corporation).

After a Monday midnight deadline passed, BP's proposed deal with Rosneft lapsed as the company failed to reach a last-minute agreement with Rosneft and resolve a dispute with BP's Russian partners in a separate joint venture, TNK-BP.

"The issue was immediately identified by staff, and after 25 minutes was resolved by our technical team, who were able to account for all the 999 calls in the system".