Sentence examples for minutes and cooled from inspiring English sources

The mixture was heated at 95 °C for five minutes and cooled at room temperature for 30 min before assembling the in vitro transcription reaction.

All reagents were prepared from analytical purity chemicals and deionized (DI) water (≥18 MΩ) that had been boiled for 30 minutes and cooled while being purged with oxygen-free nitrogen gas (C.P. grade, BOC gases).

The reaction mixture was denatured at 65°C for 10 minutes and cooled at 4°C for 5 minutes.

Mixtures were heated at 37°C for 30 minutes and cooled at 4°C for 10 minutes.

Accordingly, 5 µg of total RNA and 20 µg of random hexamers (Invitrogen) in a reaction volume of 17.3 µl were denatured at 70°C for 10 minutes and cooled on ice for 5 minutes.

Cells were pre-treated or not at 37°C for 1 hour, and then incubated with slight agitation at 50°C for 20 minutes and cooled on ice for 5 minutes.

Sometimes we would arrive in ten minutes and cool our heels; other times we would barely make it.

by heating to 95°C for 5 minutes and cooling at room temperature for 15 minutes to generate a double-stranded (ds) oligo.

The 1-hour exercise intervention, to be led by mental health professionals, will include a warm up, stretching and joint movements (15 minutes), walking (10 minutes), stepping (10 minutes), mild weight training (10 minutes) and cool down stretching (15 minutes).

Bake for 40 minutes, and cool on a cooling rack for 10 minutes before serving.

Allow to to steep foe five minutes and cool slightly before drinking.