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Time-lapse analysis of early time points of cell spreading in WT and FLNabKD cells did however suggest a slight delay in the time needed to reach the maximal area as wild-type cell spreading appeared to plateau approximately 30 minutes post-plating while the FLNabKD cells plateaued around 40 45 minutes after plating (Figure 4E).
Ten minutes after plating, unattached cells were removed by washing.
Ten to fifteen minutes after plating, the cells exit mitosis and proceed into a new cell cycle.
GIPC1 silencing significantly enhanced MDA-MB231 cell adhesion at both 30 and 60 minutes after plating (Figure S3A).
Cells were suspended, washed, and control and knockdown cells mixed before seeding onto FN-coated plates, 10 minutes after plating unattached cells were washed away and imaging was initiated 1 hour after plating.
A mix of WT and FLNabKD cells were plated on 3.5 cm dishes coated with 10 µg/ml FN and washed 30 minutes after plating then processed as above.
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We finally examined the morphological change of human synovial MSCs during a 10-minute period after plating on a culture dish.
Twenty four hours after plating, cells were fixed by incubation with 4% Paraformaldehyde for 30 minutes.
Plates were examined two and six days after plating.
After attached phage were brought to the experimental temperature, samples were taken every two minutes for up to 24 minutes, plated on LB agar plates, and the plaques were counted after 3-4 hofrs of incubation at the experimental temperature.
A few minutes after crossing the plate, Abreu received a shaving-cream pie in the face.
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