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In lambs and goats inoculated intraduodenally with C. perfringens type D culture supernatant containing ETX, nervous signs or death were observed as soon as 30 minutes after inoculation [5].
Ten minutes after inoculation, four leaf discs (6 mm in diameter) were excised from four different plants of the same genotype, floated in 20 mL distilled water for 30 min, and then transferred to a 6-well tissue culture plate with each well containing 6 mL fresh distilled water.
The anaerobic culture was maintained by sparging nitrogen for 30 minutes after inoculation.
All plants were rinsed with water five to ten minutes after inoculation.
We pressed bone cement samples in the agar 10 minutes after inoculation, after which the plates were incubated aerobically at 37° C.
Twenty μL of the samples were taken at 0, 15, 30 and 60 minutes after inoculation to enumerate the number of viable cells.
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Increase in [Ca2+]i was observed at approximate 90 minutes after the inoculation of Pst DC3000/avrRpm1 but not Pst DC3000.
Distribution of bacterial cells spread through the body of the fly was assessed by separating the fly thorax from the abdomen with fine forceps minutes after bacterial inoculation.
PDT was performed with the combination of a series of concentrations of photosensitizer polyethylenimine- (PEI-) ce6 and a series of doses of noncoherent red light 30 minutes after bacterial inoculation.
Figure 4(a) shows that the amount of viable virus released was reduced by about 1 log when the cells were treated at 30 minutes prior to inoculation, at the time of inoculation and up to 1 hour after inoculation.
We monitored the changes in [Ca2+]i during 30 45 and 80 95 minutes after the bacterial inoculation (Figure 5).
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minutes after injection
time after inoculation
hour after inoculation
minutes after plating
minutes after seeding
minutes after midnight
minutes after cross-clamping
minutes after noon
minutes after class
minutes after arrival
minutes after tracer
minutes after liftoff
minutes after takeoff
minutes after infusion
minutes after consumption
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