Sentence examples for minutes a solution from inspiring English sources

After drying the disk by passing air through it for several minutes, a solution of 7.5 mg ligand dissolved in 10 mL ethanol was introduced onto the disk and was washed with 25 mL of water.

Microarrays were hybridized at 42°C for 14 18 hours, then washed in a solution of 1.5% SDS and 1 × SSC for 5 minutes, a solution of 0.20 × SSC for 5 minutes, and two solutions of 0.05 × SSC for 10 minutes each.

The migrant cells attached to the lower surface were fixed in methanol at room temperature for 30 minutes, and stained for 20 minutes with a solution containing 0.5% crystal violet and 2% ethanol in 100 mM borate buffer (pH 9.0).

For migration and invasion-based motility assay, cells attached to the lower surface were fixed in ice-cold methanol for 10 minutes, and stained for 10 minutes with a solution containing 0.5% crystal violet and 2% ethanol in 100 mM borate buffer (pH 9.0).

After deparaffinizing and two 5-minute washes in PBS, the sections were blocked for 20 minutes with a solution of 2% unconjugated goat anti-mouse IgG in 1 x PBS.

If the stain is still there, soak the fabric for 15 minutes in a solution of: If the stain is still there, soak the fabric for 15 minutes in a solution of: 1 quart warm water.

The tissues were soaked for 30 minutes in a solution of iron nitrate (FeIII NO3 3.9H2O, 7.65 g in 1 L water).

This two-step immersion procedure was then repeated before soaking the clothes 2 or 3 minutes in a solution of aluminum sulphate (0.95 g in 1 L of water) in order to fix the dying.

Ca2+ concentration in the endosomes of fibroblast cells after incubation for 10 minutes in a solution containing 2 mM CaCl2 was 3.9±1.2 µM [17].

For antigen retrieval, OB slices were steamed for 10 minutes in a solution of 0.01 M Sodium Citrate, then immediately washed with PBS-T.

The sections went through a second PB and Triton X-100 rinse cycle and were then preincubated for 90 minutes in a solution containing 0.1 M PB and 0.5% Triton X-100, and 4% of the appropriate normal serum.