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Blots were developed by incubating the membrane for 1 minute with equal amounts of solutions A and B of Enhanced chemiluminescence (ECL) (Ornat, Rehovot, Israel).

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For maximum relaxation and productivity, she suggests bringing things down to between four and a half and six full breaths per minute, with equal-length inhalations and exhalations.

Briefly, as GLUT12 is targeted to the apical plasma membrane in high glucose conditions, in assays with a glucose gradient, cells were equilibrated for 30 minutes with equal volumes of 25 mM glucose DMEM and 2× (glucose free) uptake solution (final extracellular concentration 12.5 mM glucose).

In bulk, MyOne-Streptavidin C1 beads were washed two times for 5 minutes each with equal volume of 20 mM NaOH and three times with an equal volume of SB17T.

Upon reaching the laboratory, specimens were pooled together, decontaminated for 15 minutes with an equal volume of 4% NaOH and centrifuged at 3000 revolution per minute (rpm) for 15 minutes.

Samples were treated with RNAse A (40 mg/mL for 30 minutes at 37°C) and then extracted with equal volumes of phenol chloroform:isoamyl alcohol (25:24:1) followed by a chloroform extraction.

Wait a few minutes, and then rinse off the paste with equal parts water and vinegar to remove all residue.

Cultured epithelial cells T84 or HT29-CL19A were incubated with equal numbers of the indicated bacteria for 30 minutes and washed with HBSS.

Infected and control cells were harvested and mixed with equal amounts of 2×SDS-PAGE 2×SDS-PAGE buffer for 5 minutes.

The aqueous phase was then combined with equal volume of isopropanol, incubated at room temperature for 10 minutes and centrifuged at 12,000 g for 15 minutes at 4°C.

Briefly, sputum samples were homogenized and decontaminated with equal volume of 4% NaOH and shaken for 15 minutes at room temperature.

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