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After activating the polymerase and denaturizing DNA by heating at 95°C for 10 minutes, 40 cycles of 92°C for 15 seconds and 55°C for 1 minute were performed.
Finally, two consecutive washes (5 minutes and 1 minute) were performed with TCM199 with 20% SSS at room temperature before oocytes were incubated at 37°C for two hours preceding ICSI.
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Quantification of blood flow within the tumour (expressed in milliliter per gram per minute) was performed.
To ensure that all TBA aptamers adopted the same structure at the beginning of each experiment, a slow melting-cooling cycle at 0.2°C per minute was performed prior to each experiment.
An exercise resistance test, consisting of horizontal running for 5 min at 5 m/min, then increasing the speed of 1 m/min each minute, was performed on week 0 and after four and five weeks of treatment.
Peritoneal lavage using 3 ml PBS followed by a gentle abdominal massage for 1 minute was performed.
The critiques, each about 5 to 10 minutes, were performed by Decker, Rita Teutonico (associate professor of biotechnology, James Madison University), and Russell Kitchner (associate director and manager of graduate student career services, University of Notre Dame).
Two runs each lasting 8 minutes were performed with each run consisting of a pseudo randomized block design with 8 blocks of pictures of H, 8 blocks of U, and 8 blocks of O.
Three tests separated by at least 15 minutes were performed for each animal.
Face-to-face semi-structured interviews, lasting 45 90 minutes, were performed by the author (SM) who issued the invitations.
The interviews which lasted 15 30 minutes were performed either at the ED or the ward by handwriting of pragmatic reasons.
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