Seven datasets (soaking HI-6 for 1, 2, 3, 3, 4, 5, and 10 minutes) were collected for HI-6sarinnonaged-mAChE, four datasets were collected for K027mAChE, and three datasets were collected for HI-6BRmAChE.
The supernatant (12,000 rpm for 10 minutes) was collected and injected (20 µl) into an HPLC system equipped with a fluorescence detector (Waters, MA).
Air (200 L in 2 minutes) was collected by air sampler MBASS30LKS100 and conducted over microbiological plates for culturing.
One minute fractions were collected.
Cells were spun at 4°C, 5000 rpm for 5 minutes, supernatants were collected and re-spun at 4°C, 14,000 rpm for 25 minutes.
Here, two consecutive fMRI experiments of 3'40″ minutes each were collected in all n = 18 subjects.
The next day, samples were centrifuged at 14,000 rpm at room temperature for 5 minutes, supernatants were collected and transferred to a 96-wells polypropylene plate.
Following centrifugation at 20,000×g for 15 minutes, supernatants were collected and protein concentration was determined using the bicinchoninic acid protein assay (BCA).
After 3 minutes, cells were collected by centrifugation.
Fractionation occurred at a flow rate of 6.3 mL/hr at 4°C, and 30-minute fractions were collected.
