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There are 10 minute washes between each contraction.
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Blots were exposed to Kodak x-ray film for 18 hours to 15 days between washes and were stripped for re-probing using two fifteen minute washes of boiling 0.1 × SSC/0.1% SDS.
Both tests were performed at this identical speed with a 10 minute "wash-out" period in between.
Transfer and blot accordingly by incubating the target protein primary antibody and associated secondary to each blot with at least four, 3-minute wash steps between each incubation.
Three 5-minute washes with PBS were carried out between all incubation steps.
To remove the unbound protein, plate was washed thrice, 5 minute per wash, with wash buffer.
Between the incubations, 3 times 2 minutes washing steps in PBS were performed.
Between the incubation steps, 3 times 2 minutes washing steps in PBS were performed.
It has a 20-minute wash for 1.5kg.
A 5×20 minutes washing protocol (PBT) was followed by final washing steps in PBS (5×20 minutes).
After ten minutes, wash off.
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