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In order to obtain the optimum condition, the effect of deposition time was investigated and a duration time of 10 minute was selected as an optimum condition.
A heating ramp of 5 °C per minute was selected to expose the cylindrical specimens to target temperatures up to 800 °C.
A heating rate of 5 °C per minute was selected, which is very close to the RILEM recommended heating rate (RILEM TC 129-MHT 1995).
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Forty minutes was selected because this corresponds to a good 10-km time and a 10-km pace is generally associated with lactate threshold.
Hence a time point of 30 minutes was selected for checking intracellular accumulation of all these three substrates in the mutant variants and then compared with host AD1-8u.
Exposure of the immobilized complex to 1150 mM salt for 15 minutes was selected as an effective and experimentally repeatable (Fig. 1B D) assay protocol that liberated a significant fraction of the protein members (Fig. 2, 3).
The meal duration of 30 minutes was selected to simulate high-intensity eating during which mastication and swallowing were continuously repeated, similar to the situation when a patient eats in a continuous fashion with the support of a meal assistant.
Therefore a total yield of 44.3% of DM (69.0% of the theoretical maximum, equivalent to over a 6-fold increase from raw material) by LHW pretreatment at 190°C for 10 minutes was selected for further experiments.
A LHW pretreatment at 190°C for 10 minutes was selected as the optimal condition for maximising sugar release which reached 69% of the theoretical maximum after 72 hours of saccharification.
Five weeks after the unilateral 6-OHDA MFB lesion procedure, animals were grouped based on apomorphine (0.05 mg/kg, s.c).-induced rotational behaviour: animals with >300 rotations per 60 minutes were selected.
The metabolites with the highest absolute regression coefficients per time point (thus 0, 15, 30, 45, 60, 90, 120 and 180 minutes) were selected from the LC-MS polar and GC-MS global n-PLS-DA models as being most discriminative between subjects treated with diclofenac and placebo.
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