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Quantification of blood flow within the tumour (expressed in milliliter per gram per minute) was performed.
To ensure that all TBA aptamers adopted the same structure at the beginning of each experiment, a slow melting-cooling cycle at 0.2°C per minute was performed prior to each experiment.
An exercise resistance test, consisting of horizontal running for 5 min at 5 m/min, then increasing the speed of 1 m/min each minute, was performed on week 0 and after four and five weeks of treatment.
Peritoneal lavage using 3 ml PBS followed by a gentle abdominal massage for 1 minute was performed.
The single-step RT-PCR (consisting of a 10-minute RT step at 45°C and 10 minutes of Taq polymerase activation at 95°C followed by 40 cycles of PCR at 95°C for 15 seconds and at 60°C for 1 minute) was performed on an ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA).
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After activating the polymerase and denaturizing DNA by heating at 95°C for 10 minutes, 40 cycles of 92°C for 15 seconds and 55°C for 1 minute were performed.
Finally, two consecutive washes (5 minutes and 1 minute) were performed with TCM199 with 20% SSS at room temperature before oocytes were incubated at 37°C for two hours preceding ICSI.
The bronchoscopy, which took about 20 minutes, was performed by a team that included two physicians and two technical support staff.
The operation, which took seven minutes, was performed, with local anesthetic, at the kitchen table of Sklar's loft, in Brooklyn.
Two years ago I witnessed a different kind of theatrical experiment, in which Shakespeare's "Much Ado About Nothing," in the original language, trimmed to 90 minutes, was performed before an audience largely unfamiliar with Shakespeare: inmates at Rikers Island.
The whole treatment lasting 20 minutes was performed in protective Ar atmosphere under 500 rpm rotational speed of planet carrier.
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CEO of Professional Science Editing for Scientists @ prosciediting.com