Sentence examples for minute was applied from inspiring English sources

A second dose of UV light (1 minute) was applied to covalently link RGDS within the hydrogel.

One hundred percent oxygen with high flow (5 liters per minute) was applied at least one minute before intubation or LMA insertion and immediately after extubation.

When a current density of 3200 mA g−1 (i.e., one complete cycle in one minute) is applied, the array delivers a reversible capacity of 42 mAh g−1.

A moving average of 30 minutes was applied to smooth the data.

A final wash with Buffer RPE for 2 minutes was applied before the RNA was eluted in 30 60 µl of RNase-free water.

Neurons were then incubated for 30 minutes, washed by conditioned neurobasal medium and then 100 µM NMDA for 3 minutes was applied to stimulate the extrasynaptic population of NMDA receptors.

The clear supernatant obtained after centrifugation at 220000× g for 35 minutes was applied to a monomeric avidin-Sepharose column (5 ml bed volume), equilibrated with 50 mM Tris-HCl buffer, pH 8.0 containing 50 mM NaCl, 0.5% Tween 20 and 0.05% Brij58.

Gentle centrifugation (1800 rpm, 6 minutes) was applied to remove any remaining plasma.

Vecstatin ABC reagent (Vector) and DAB reagent (Sigma) for 2 10 minutes was applied.

A gradient of 12%to30%0% acetonitrile (40 minutes) was applied over a two-hour run for peptide separation.

A thermal treatment (90°C, 15 minutes) was applied to the inoculum to eliminate any methanogenic activity [ 17, 26].