Exact(2)
The survivals of the S8 E30 minute treated group showed less injury at 6 hours and 24 hours as compared with nontreated groups and S8 L6 hour treated animals.
Nausea was reported more frequently in patients with moderate or severe renal impairment (eGFR <60 mL per minute) treated with liraglutide compared with those with normal or mild renal impairment, and the effect was dose-dependent.
Similar(58)
I pull up my phone's stopwatch to time him for a minute, treating each potato as a lap: his slowest is 10.7 seconds, his quickest 6.4.
Ten millilitres of the sample was divided into two aliquots of 5 ml each; one aliquot was incubated at 50°C in a water bath for 30 minutes (treated sample) and the second aliquot remained at room temperature (non-treated sample).
As indicated, prior to addition of isoproterenol some cells were: supplemented with 50 mM NaHEPES (pH 7) and maintained at 4°C for 15 minutes; supplemented with 400 mM sucrose for 15 minutes; treated with either dynasore (80 µM) or vehicle (DMSO) for 30 minutes.
For this purpose, biotinylated HEK293-uPAR cells were incubated at 37°C for 20 minutes, treated with GSH to remove cell-surface biotin, reincubated at 37°C for 10 or 20 minutes, and the amount of cell-surface (recycled) uPAR evaluated after a second GSH treatment by immunoprecipitation with anti-uPAR antibodies and immunoblotting with HRP.
Three samples, namely, untreated and 5- and 15-minute treated were taken for ATR-FTIR analysis.
Cells were then fixed in 2% formaldehyde for 5 minutes, treated with 20% isopropanol for 0.5 minutes, and stained with Oil Red O for 20 minutes.
After incubation in dark for 30 minutes, treated cells were analyzed by FACS caliber and the obtained results were analyzed by the Cell Quest software.
Following BrdU (BD Biosciences) incorporation, cells were fixed with 4% PFA for 10 minutes, treated with 95% methanol for 10 minutes, permeabilized with 2 N HCl for 10 minutes, and neutralized twice with 0.1 M sodium borate for 5 minutes each.
After the migration period, cells were fixed with ice cold 70% ethanol for 10 minutes, treated with 0.1% TritonX-100 in PBS for 2 minutes and stained with haematoxylin for 10 minutes.
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