It took fifteen minutes to stabilize her blood pressure.
"For us it's difficult to implement that 'golden hour,' " said Maj. Gen. Mussa Wardak, director of the army's five military hospitals, referring to the critical early minutes to stabilize and treat trauma victims.
After 24 h incubation with RBV, 100 µl of CellTiter-Glo reagent was added to each well, plates were mixed for 2 minutes on orbital shaker to induce cell lysis, and incubated for 10 minutes to stabilize the luminescence signal.
Briefly, cells were incubated with CellTiter-Glo Reagent and mixed on an orbital shaker for 2 minutes to induce cell lysis and incubated at room temperature for 10 minutes to stabilize luminescent signal.
At baseline, patients breathed slowly for a minimum of 5 minutes to stabilize resting gas measurements.
Cells were then lysed by shaking in an orbital shaker for 2 minutes, followed by incubation at room temperature for 10 minutes to stabilize the luminescent signal.
In pretest session, each subject was required to sit down and relax for 10 minutes to stabilize his/her blood pressure.
The plates were incubated at room temperature for 10 minutes to stabilize luminescence signals and then the stabilized signals were quantified with an Orion Luminometer (Berthold Detection Systems, Oak Ridge, TN, USA).
After the attached phage and cells were added to the temperature-equilibrated media, four and eight minute samples of 500 μl were added directly to 1 ml of RNAprotect™ Bacteria Reagent (Qiagen Cat. No.76506) and incubated at room temperature for 5 minutes to stabilize the RNA.
Before the start of the experiment with new fuel variant, the fuel used in the previous experiment was completely purged from the fuel line, filter, fuel pump, and fuel tank and the engine was left to operate for about three minutes to stabilize in its new condition.
