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Then a substrate solution (tetra-methylbenzidine) was pipette and incubated for 15 minute to induce development of color.
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A smoker would theoretically have to consume nearly 1,500 pounds of marijuana within about 15 minutes to induce a lethal response.
Following 24 hours of treatment, animals in inhibitor were placed at 33°C for 90 minutes to induce heat shock.
Sperm was harvested from the cauda epididymis of male donor mice using forceps, placed in 300ul of HTF media, and incubated for 30 minutes to induce capacitation.
Briefly, cells were incubated with CellTiter-Glo Reagent and mixed on an orbital shaker for 2 minutes to induce cell lysis and incubated at room temperature for 10 minutes to stabilize luminescent signal.
We monitored luciferase reactivation in strains A1-A8 by shifting exponentially growing cells from 30°C to 37°C for 30 minutes to induce expression of heat shock proteins, including Hsp104, and then to 42°C to cause extensive aggregation of luciferase.
Diluted cultures were incubated with shaking at 37°C for 30 minutes to induce expression of heat shock proteins, and then transferred to a 42°C water bath and incubated with shaking for one hour.
The positive controls were treated with DNAse for 10 minutes to induce DNA strand breaks.
The contents were mixed for 2 minutes to induce cell lysis.
Fifty microliters of reconstituted reagent was added to an equal volume of cell suspension and then mixed in a shaker for 2 minutes to induce cell lysis.
Overnight cultures were diluted to an OD600nm = 0.2 into the same selective medium and incubated at 37°C shaking for 30 minutes to induce expression of heat shock proteins.
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