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Continue stirring for one minute to activate the gluten in the flour.
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Let it stand for 15 minutes to activate.
On a clean, floured surface, knead the dough for 5 minutes to activate the yeast and stretch the glutens within it.
The best news is that, unlike the female hormonal contraceptive pill which takes a week to kick in the compound might take only hours or minutes to activate.
Cells were scraped into 10 mM MnCl2, 50 mM Tris-HCl, pH 7.5 and heated to 56°C for 10 minutes to activate arginase.
The reactions were incubated at 95°C for 5 minutes to activate the FastStart Taq DNA polymerase, followed by 45 cycles at 95°C for 10 seconds, 60°C for 10 seconds, and 72°C for 8 seconds.
The first-round amplification reactions began with a "hot start" at 95°C for 10 minutes to activate the polymerase, followed by 30 cycles for 30 seconds at 94°C, 30 seconds at 55°C, and 1.5 minutes at 72°C, then a step at 7 minutes at 72°C was followed to end the cycles.
cDNA was denatured at 95°C for 5 minutes to activate the Hot-start Taq DNA polymerase.
The thermal cycling profile for both amplicons began with a 95°C incubation for 10 minutes to activate the AmpliTaq Gold DNA polymerase.
Cells were incubated with and without TNF (6 ng/mL) or with IL-1β (1 ng/mL) (Sigma-Aldrich, Milano, Italy) for 10 minutes to activate signaling pathways.
The thermal cycling profile for both amplicons started with a 95°C incubation for 3 minutes to activate the hot-start iTaq DNA polymerase.
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