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Cells were fixed at 15 minute time points after nocodazole removal and scored for bi-orientation.
Separately, inhibition of JNK or ERK prevented subsequent additional permeability increases as stretch continued to 60 minute time points.
In contrast, ligand internalization was significantly delayed in the truncated Δ716 G-CSFR (p<0.05 for 30-120 minutimeime points, compared to WT).
The percent of receptor remaining on the cell surface was determined by normalizing the 5, 15, 30 and 60 minute time points to the 0 min time point for the corresponding transfection.
Staining with Hoechst showed that the DNA became highly compact and apparently took up much less space in each cell (compare 15 and 30 minute with 0 minute time points, Figure 7A).
Figures 1A and IC show that a subset of substrate peptides displays increased phosphorylation at 1, 5, 10, 20, 30, 45, 60 & 120 minute time points, when incubated with 5 µg/ml of MAP3K8.
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In the placebo treatment, a capsule filled with maltodextrin was administered before the exercise protocol and at the 90 minute time point.
Significance labelling represents a significant posthoc comparison between placebo versus caffeine and NDRI at the 165 minute time point through exercise.
Results RIJV diameter increased with 30° PLR maneuver, more prominent after 1 minute in comparison to 10th minute time point.
PP administration, we also looked at a 90 minute time point.
Reactions were started by adding enzyme after spotting the 0 minute time point.
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